Download MendeleyCryo-EM structure of a Legionella effector complexed with actin and AMP
Zhou, X.T., Wang, X.F., Tan, J.X., Zhu, Y.Q.To be published.
8JO3
Cryo-EM structure of a Legionella effector complexed with actin and AMP
- PDB DOI: https://doi.org/10.2210/pdb8JO3/pdb
- EM Map EMD-36454: EMDB EMDataResource
- Classification: TOXIN
- Organism(s): Legionella sainthelensi, Oryctolagus cuniculus
- Expression System: Escherichia coli BL21
- Mutation(s): No
- Deposited: 2023-06-07 Released: 2024-05-01
- Funding Organization(s): National Natural Science Foundation of China (NSFC), Ministry of Science and Technology (MoST, China)
Experimental Data Snapshot
- Method: ELECTRON MICROSCOPY
- Resolution: 2.66 Å
- Aggregation State: PARTICLE
- Reconstruction Method: SINGLE PARTICLE
wwPDB Validation 3D Report Full Report
This is version 1.0 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Substrate of the Dot/Icm secretion system | 436 | Legionella sainthelensi | Mutation(s): 0 Gene Names: CAB17_03485 |  | |
UniProt | |||||
Find proteins for A0A2H5FI52 (Legionella sainthelensi) Explore A0A2H5FI52 Go to UniProtKB: A0A2H5FI52 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A0A2H5FI52 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Actin, alpha skeletal muscle | 377 | Oryctolagus cuniculus | Mutation(s): 0 EC: 3.6.4 |  | |
UniProt | |||||
Find proteins for P68135 (Oryctolagus cuniculus) Explore P68135 Go to UniProtKB: P68135 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P68135 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| ATP Query on ATP | E [auth B] | ADENOSINE-5'-TRIPHOSPHATE C10 H16 N5 O13 P3 ZKHQWZAMYRWXGA-KQYNXXCUSA-N |  | ||
| AMP (Subject of Investigation/LOI) Query on AMP | C [auth A] | ADENOSINE MONOPHOSPHATE C10 H14 N5 O7 P UDMBCSSLTHHNCD-KQYNXXCUSA-N |  | ||
| CA Query on CA | D [auth B] | CALCIUM ION Ca BHPQYMZQTOCNFJ-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: ELECTRON MICROSCOPY
- Resolution: 2.66 Å
- Aggregation State: PARTICLE
- Reconstruction Method: SINGLE PARTICLE
| Task | Software Package | Version |
|---|---|---|
| MODEL REFINEMENT | PHENIX | dev_4694: |
Entry History & Funding Information
Deposition Data
- Released Date: 2024-05-01 Deposition Author(s): Zhou, X.T., Wang, X.F., Tan, J.X., Zhu, Y.Q.
| Funding Organization | Location | Grant Number |
|---|---|---|
| National Natural Science Foundation of China (NSFC) | China | 81925024 |
| National Natural Science Foundation of China (NSFC) | China | 21974002 |
| National Natural Science Foundation of China (NSFC) | China | 22174003 |
| Ministry of Science and Technology (MoST, China) | China | 2017YFA0503900 |
Revision History (Full details and data files)
- Version 1.0: 2024-05-01
Type: Initial release
