8R6W

Structure of the SFTSV L protein in a transcription-priming state with bound capped RNA [TRANSCRIPTION-PRIMING]

PDB DOI: https://doi.org/10.2210/pdb8R6W/pdb
EM Map EMD-18967: EMDB EMDataResource
NAKB: 8R6W

Classification: VIRAL PROTEIN
Organism(s): SFTS virus AH12
Expression System: Trichoplusia ni
Mutation(s): Yes

Deposited: 2023-11-23 Released: 2024-04-24
Deposition Author(s): Williams, H.M., Thorkelsson, S.R., Vogel, D., Busch, C., Milewski, M., Cusack, S., Grunewald, K., Quemin, E.R.J., Rosenthal, M.
Funding Organization(s): Leibniz Association, German Research Foundation (DFG), German Federal Ministry for Education and Research

Experimental Data Snapshot

Method: ELECTRON MICROSCOPY
Resolution: 3.35 Å
Aggregation State: PARTICLE
Reconstruction Method: SINGLE PARTICLE

wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Literature

Structural snapshots of phenuivirus cap-snatching and transcription

Williams, H.M., Thorkelsson, S.R., Vogel, D., Busch, C., Milewski, M., Cusack, S., Grunewald, K., Quemin, E.R.J., Rosenthal, M.

(2024) Nucleic Acids Res

DOI: https://doi.org/10.1093/nar/gkae330

Macromolecule Content

Total Structure Weight: 261.49 kDa
Atom Count: 16,539
Modelled Residue Count: 2,015
Deposited Residue Count: 2,164
Unique protein chains: 1
Unique nucleic acid chains: 4

Macromolecules

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(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
RNA-directed RNA polymerase L	A	2,084	SFTS virus AH12	Mutation(s): 1
UniProt
Find proteins for U3GU88 (Dabie bandavirus) Explore U3GU88 Go to UniProtKB: U3GU88
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	U3GU88
Sequence Annotations Expand
Reference Sequence

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Entity ID: 2
Molecule	Chains	Length	Organism	Image
RNA (5'-R(APCPAPC)-3')	B [auth G]	16	SFTS virus AH12
Sequence Annotations Expand
Reference Sequence

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Entity ID: 3
Molecule	Chains	Length	Organism	Image
RNA (5'-R(APCPAPCPAPGPAPGPAPCPGPCPCPCPAPG)-3')	C [auth P]	20	SFTS virus AH12
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Reference Sequence

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Entity ID: 4
Molecule	Chains	Length	Organism	Image
RNA (5'-R(PCPUPGPGPGPCPGPGPUPCPUPUPUPGPUPGP*U)-3')	D [auth T]	26	SFTS virus AH12
Sequence Annotations Expand
Reference Sequence

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Entity ID: 5
Molecule	Chains	Length	Organism	Image
RNA (5'-R((M7G)APAPA)-3')	E [auth C]	18	SFTS virus AH12
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: ELECTRON MICROSCOPY
Resolution: 3.35 Å
Aggregation State: PARTICLE
Reconstruction Method: SINGLE PARTICLE

EM Software:

Task	Software Package	Version
MODEL REFINEMENT	PHENIX
RECONSTRUCTION	RELION

Structure Validation

View Full Validation Report

Entry History & Funding Information

Deposition Data

Released Date: 2024-04-24

Deposition Author(s):

Funding Organization	Location	Grant Number
Leibniz Association	Germany	K72/2017
German Research Foundation (DFG)	Germany	NST 152/772-1, 774-1, 775-1 and 776-1
German Federal Ministry for Education and Research	Germany	01KI2019